財團法人國家實驗研究院國家實驗動物中心

HOME > 種原資料庫 > RMRC13108

RMRC No.

RMRC13108

Order

Strain/Stock Designation

B6.129-Mcl-1^tm1(130)Yen

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos) 　Industry：11,105NTD　Academia: 7,775NTD　NSTC: 5,415NTD
(Cryo Recovery) 　Industry：20,815NTD　Academia: 13,880NTD　NSTC: 13,880NTD

Last Update Date

2015/12/18

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

MSK (mutant stock),CON (congenic strain)

Mutant Type

：

TM (Target Mutation)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

CD8 T cells hypomorph, floxed Two upstream promoter elements located at positions -87 (the SIE site) and -70 (the CRE-2 site) were targeted for mutation. The SIE and CRE-2 sites were replaced with BamHI and EcoRI restriction site, respectively, and a floxed neomycin resistance cassette was placed downstream of exon 1. Founder mice were crossed with EIIa-Cre transgenic mice to remove the neomycin cassette. Immunoblot analysis revealed gene expression was reduced by 90% in the thymus but was marginally or not at all affected in various other major organs examined, including liver, heart, lung and spleen. The altered expression was attributed to the lack of SIE and CRE-2 sites because expression was not disturbed in the thymus of mice homozygous for the same loxP insertion but with intact promoter elements.

Depositor

：

Hsin-Fang Yang-Yen

楊性芳

：

Allele Symbol	：	Mcl-1^tm1(130)Yen

Allele Name	：	myeloid cell leukemia sequence 1, target mutation 1, Hsin-Fang Yang-Yen

Allele Description	：






Strain of Origin	：	129

Gene Symbol	：	Mcl-1

Gene Name	：	myeloid cell leukemia sequence 1

Chromosome	：	3






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Apoptosis	Cancer
Immunology and Inflammation

Construction Technologies

：

Cre-lox system

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x wild type

References

：

PubMed ID: 19234191

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： J. Immunol. 182(5):2959-2968 (2009)

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： J. Immunol. 182(5):2959-2968 (2009)

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Hsin-Fang Yang-Yen所提供

Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Hsin-Fang Yang-Yen

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email:

Construct Map

：