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RMRC No. RMRC13397 Order
Strain/Stock Designation C57BL/6-Phrf1tm1Cms/Narl
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　Industry：11,105NTD　Academia: 7,775NTD　NSTC: 5,415NTD
(Cryo Recovery) 　Industry：20,815NTD　Academia: 13,880NTD　NSTC: 13,880NTD
Last Update Date 2023/8/21
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： COI (coisogenic strain),MSR (mutant strain)
Mutant Type ： TM (Target Mutation)
Other Names ：
Background Strain ： C57BL/6
Strain Description ：
1. 
loxP elements were introduced to flank exon 2 and exon 9 (a.a. 1-343) of the murine Phrf1 gene by using CRISPR/Cas9 technology.
2. 
Forebrain-specific Phrf1 knockout mice could be producing by crossing this strain with Camk2a-iCre transgenic mice. The phenotype of forebrain-specific Phrf1 knockout mice is as follows,
     (a) exhibited similar anxiety like behaviors 
     (b) impaired spatial learning and memory
     (c) dendritic structures of CA1 pyramidal neurons are altered, including decrease of the                  total dendritic length and branches, the reduction of internodal segment length...etc
3. 
Phrf1 is also involved in acute promyelocytic leukemia (APL) models and modulating non-homologous end-joining (NHEJ)
Depositor ：
Mau-Sun Chang / 張茂山
Email ： mschang@ntu.edu.tw
Allele Symbol ： Phrf1tm1Cms
Allele Name ： PHD and ring finger domains 1, target mutation 1, Mau-Sun Chang
Allele Description ：
Strain of Origin ： C57BL/6
Gene Symbol ： Phrf1
Gene Name ： PHD and ring finger domains 1
Chromosome ： 7
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Cancer Neurobiology Research
Pharmacology and Toxicology
Construction Technologies ：
Cre-lox system
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： homo x hetero/hemi
References ： PubMed ID: 32616804

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： Shih et al., Sci. Rep. 2020. 10. 10857
Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： Shih et al., Sci. Rep. 2020. 10. 10857
Construct Map ：
 
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