財團法人國家實驗研究院國家實驗動物中心

HOME > 種原資料庫 > RMRC13223

RMRC No.

RMRC13223

Order

Strain/Stock Designation

C57BL/6- Tg (SEPT12-Flag/SEPT12/GFP)25Kpln

Status

EM| (Cryopreserved Embryos)

Availability

Level4

Pricing

(Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD

Last Update Date

2017/2/15

Analysis Data

Description
Genes &
alleles
Phenotype
Health &
husbandry
References
Specific Term
and Conditions
Construct map

Species

：

laboratory mouse

Strain Type

：

COI (coisogenic strain),MSR (mutant strain)

Mutant Type

：

TG (Transgenic)

Other Names

：

Background Strain

：

C57BL/6

Strain Description

：

1.Core promoter region of human SEPT12 was approximately 2kb and located in upstream of Exon1 encompassing Exon1 and Exon2.
2. Full length cDNA of human SEPT12 was fused with N-terminal Flag and C-terminal GFP.
3. The testis-specific expression of SEPT12-GFP was discovered in this strain.
4. SEPT12-GFP was located at the sperm annulus in spermatozoa.
5. C57BL/6J background

Depositor

：

Pao-Lin Kuo

郭保麟

：

paolinkuo@gmail.com

Allele Symbol	：	Tg (SEPT12-Flag/SEPT12/GFP)25Kpln

Allele Name	：	Transgenic insertion 25, Pao-Lin Kuo

Allele Description	：






Strain of Origin	：	C57BL/6

Gene Symbol	：	SEPT12

Gene Name	：	septin 12

Chromosome	：	UN






Genotyping Method	：	Download
Genotyping NLAC	：

Research Application

：

Reproductive Biology

Construction Technologies

：

fluorescent proteins

Animal Health Reports	：

Colony Maintenance Breeding & Husbandry	：

Colony Maintenance Mating System	：	hetero/hemi x wild type

References

：

PubMed ID: 24213608

Specific Terms of Use

：

有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.

分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應引用下述已發表之論文： SEPT12-microtubule complexes are required for sperm head and tail formation.

Should the use of this material result in one or more scientific
publication(s) the recipient agrees to cite the paper(s)： SEPT12-microtubule complexes are required for sperm head and tail formation.

分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: paolinkuo@gmail.com

Construct Map

：