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RMRC No. RMRC13272 Order
Strain/Stock Designation NOD.CB17-Prkdcscid -Tg(U6-RNAi:Il27, Ubi-eGFP)Sytwu
Status
EM| (Cryopreserved Embryos)
Availability Level4
Pricing (Embryos) 　International：560USD
(Cryo Recovery) 　International：760USD
Last Update Date 2016/5/26
Analysis Data
  • Description
  • Genes &
    alleles
  • Phenotype
  • Health &
    husbandry
  • References
  • Specific Term
    and Conditions
  • Construct map
Species ： laboratory mouse
Strain Type ： MSK (mutant stock),CON (congenic strain)
Mutant Type ： TG (Transgenic),TM (Target Mutation)
Other Names ：
Background Strain ： NOD.CB17
Strain Description ：
1.  Il27 shRNA and eGFP driven by U6 promoter and Ubi promoter respectively are constructed in same plasmid.
2.  The plasmid carried two transgenes is microinjected into NOD embryos.
3.  This strain is derived from NOD-Tg(U6-RNAi:Il27, Ubi-GFP)Sytwu mated with NOD.CB17-Prkdcscid/J.

Depositor ：
Huey-Kang Sytwu / 司徒惠康
Email ： sytwu@ndmctsgh.edu.tw
Allele Symbol ： Tg(U6-RNAi:Il27, Ubi-eGFP)Sytwu
Allele Name ： Transgenic insertion, Huey-Kang Sytwu
Allele Description ：
Allele Symbol ： Prkdcscid/J
Allele Name ： protein kinase, DNA activated, catalytic polypeptide; severe combined immunodeficiency
Allele Description ： A T-to-A transversion point mutation at a position corresponding to codon 4095 created a premature stop codon.
Strain of Origin ： NOD/Sytwu
Gene Symbol ： Il27
Gene Name ： interleukin 27
Chromosome ： UN
Gene Symbol ： Prkdc
Gene Name ： protein kinase, DNA activated, catalytic polypeptide
Chromosome ： 16
Genotyping Method ： Download            
Genotyping NLAC ：            
Research Application ：
Immunology and Inflammation Diabetes and Obesity
Virology
Construction Technologies ： No
Animal Health Reports ：
Colony Maintenance
Breeding & Husbandry 		：
Colony Maintenance
Mating System 		： hetero/hemi x wild type
References ： No

Specific Terms of Use ：
有特別規定
本生物材料僅限使用於學術研究。寄存者對於分讓申請人使用本材料 所獲得之研究成果，不主張任何專利、智慧財產權及其他財產權
DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of BIOLOGICAL RESOURCE for academic research purpose.
分讓申請人公開發表使用本材料所獲得之論文或其他研究成果時，應揭露並致謝本材料係由Dr. Huey-Kang Sytwu所提供
Should the use of this material result in one or more scientific publication(s) the Recipient Investigator agrees to mention and acknowledge Dr. Huey-Kang Sytwu
分讓申請人向國研院動物中心國家實驗鼠種原庫(NLAC-RMRC)申請本材料時，應附上寄存人簽名之授權分讓同意書作為附件；NLAC- RMRC 在取得寄存人書面同意後，始進行此材料之分讓。
The recipient should obtain a written consent from the depositor of the BIOLOGICAL RESOURCE, prior to NLAC-RMRC accepting the material distribution request.
Depositor Email: sytwu@ndmctsgh.edu.tw
Construct Map ：
 
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